2. General Description
2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 45
2.5.8 AlphaScreen/AlphaLISA System (Infinite F200 PRO only)
For uncompromised performance, the Infinite F200 PRO uses its dedicated
fluorescence top detection module (see chapter 2.5.2) for
AlphaScreen/AlphaLISA measurements. The AlphaScreen/AlphaLISA module
utilizes the instrument’s flash lamp as a light source in combination with a
680(30) nm excitation filter and dedicated emission filters for AlphaScreen and
AlphaLISA, respectively. A dichroic mirror optimized for the AlphaScreen and
AlphaLISA wavelengths serves to maximize the excitation and emission light
intensities and is selected automatically for Alpha measurements.
Note
AlphaScreen/AlphaLISA measurements are only possible as endpoint
measurements in white or light gray microplates and cannot be
performed in combination with the injector system and the heating
system.
AlphaScreen/AlphaLISA Mirror
The Infinite F200 PRO with AlphaScreen/AlphaLISA option is equipped with a
dichroic mirror optimized for Alpha assays. The mirror is selected automatically
for all Alpha measurements.
Mirror type Reflection (Excitation) Transmission (Emission)
650 dichroic (AlphaScreen/AlphaLISA)
665 – 700 nm 540 – 635 nm
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