tecan Infinite 200 PRO - 7.3.6 Absorbance Accuracy; 7.3.7 Absorbance Wavelength Accuracy

7. Quality Control
170 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04
Calculation of the Sensitivity (Detection Limit):
15
BATP
B
8
e1
1
*0002.0*
meanmean
Stdev*3*102
)well/fmol(imitDetectionL
−
−
−
⋅
=
2*10
-8
Concentration of ATP standard [M]
Stdev
B
Standard deviation of Blank
mean
ATP
Average of wells filled with ATP standard
mean
B
Average of Blank wells
0.0002 Conversion into mol/well
1/1e
-15
Conversion into fmol/well
The result of the formula determines the detection limit in fmol/well.
7.3.6 Absorbance Accuracy
Use MultiCheck Plate – For details please refer to the MultiCheck Instructions for
Use.
7.3.7 Absorbance Wavelength Accuracy
The wavelength accuracy defines the deviation of the set measurement
wavelengths from the nominal wavelength. This test is only valid for the Infinite
M200 PRO.
Measurement Parameters:
Parameter Setting
Reading Mode Absorbance Scan
Measurement Wavelength from/to 300 – 850 nm
Step Size 1 nm
Number of flashes 25
Settle Time 0
Plate Type MultiCheck plate
Material/Reagents:
MultiCheck plate
Calculation of Wavelength Accuracy:
Please refer to the data sheet in the instructions for use for your MultiCheck plate.
mt
MaxMaxAccuracyWavelength −=
Max
t
theoretical maximum
Max
m
measured maximum