tecan Infinite 200 PRO - Page 162

7. Quality Control
162 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04
Material/Reagents:
25 nM Fluorescein (in 0.01 M NaOH) (Fluorescein sodium salt, Sigma F6377)
0.01 M NaOH (=Blank) (NaOH pellets, Merck article no. 6495 or Sigma S8045)
1 Greiner 96-well plate µClear, black with transparent bottom
200 µl Pipette + tips
Calculation of Detection Limit (Sensitivity):
See 7.3.1 Fluorescence Top: Sensitivity.
Sensitivity (Enhanced)
Perform the following measurement to determine the detection limit for
Fluorescein:
Measurement Parameters:
Parameter Setting
Reading Mode Fluorescence Bottom
Ex Wavelength Infinite F200 PRO: 485 (20) nm
Infinite M200 PRO: 485 nm
Em Wavelength Infinite F200 PRO: 535 (25) nm
Infinite M200 PRO: 535 nm
Number of flashes 25
Integration Time 40
Settle Time 0
Gain Optimal
Plate Type GRE96fb
Plate Layout
Pipette 200 µl of 1 nM fluorescein or the blank solution (0.01 M NaOH) into the
appropriate wells according to the plate layout:
<> 1 2 3 4 5 6 7 8 9 10 11 12
A
1 nM Fluorescein Blank 1 nM Fluorescein Blank 1 nM Fluorescein Blank 1 nM Fluorescein Blank 1 nM Fluorescein Blank 1 nM Fluorescein Blank
B
C
D
E
F
G
H