tecan Infinite 200 PRO - Page 175

7. Quality Control
2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 175
Measurement Parameters:
Parameter Setting
Reading Mode AlphaScreen/AlphaLISA
Ex Wavelength 680 (30) nm
Em Wavelength 570 (100) nm
Excitation Time 1000 ms
Integration Time 500 ms
Gain Calculated from Well: A1
Plate Type GRE384fw
Evaluation:
Calculate the average and standard deviation for each Omnibead concentration.
Perform a blank reduction by subtracting the average signal of the blanks wells
from the average signal of each Omnibead concentration.
Plot the average blank-corrected values against the final Omnibead
concentrations in a XY scatter diagram. Add a linear trend line with intercept set
to 0 and solve the trend line equation (y = kx) using the 3-fold standard deviation
of the blank as y.
k
y
x =
y 3* stdev of the blank (wells N2-P6)
Extrapolate the detection limit [ng/ml] by using the 3-fold standard deviation of the
blank as y.