tecan Infinite 200 PRO - Page 6

6 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04
4.1 Safety ................................................................................................... 53
4.2 Gas Control Module Features............................................................ 54
4.2.1 Gas Control Module Configurations ...................................................... 54
4.2.2 Top and Rear Views of the Gas Control Module .................................. 55
4.3 Main Menu of Modes .......................................................................... 57
4.3.1 CO2 Mode ............................................................................................ 57
4.3.2 O2 Mode ............................................................................................... 58
4.3.3 DUAL Mode .......................................................................................... 59
4.3.4 Manual Mode ........................................................................................ 60
4.4 Settings Menu ..................................................................................... 61
4.5 Installing the Gas Control Module .................................................... 62
4.5.1 Requirements ....................................................................................... 62
4.5.2 Installation Procedure ........................................................................... 63
4.6 Operating the Gas Control Module ................................................... 67
4.7 CO
2
and N
2
Gas Cylinders (Not Supplied Accessory) ..................... 71
4.8 Troubleshooting the Gas Control Module ........................................ 72
5. Operating the Instrument ............................................................................... 75
5.1 Introduction......................................................................................... 75
5.2 General Operating Features .............................................................. 76
5.2.1 Instrument Start Up .............................................................................. 76
5.3 General Options .................................................................................. 76
5.4 Defining Filter Slides (Infinite F200 PRO only) ................................. 78
5.4.1 About Filters ......................................................................................... 78
5.4.2 Filter Slide and Filter Orientation .......................................................... 78
5.4.3 Installing a Custom Filter ...................................................................... 80
5.4.4 Defining the Filters ................................................................................ 83
5.5 Optimizing Fluorescence Measurements ......................................... 87
5.5.1 Instrument Parameters ......................................................................... 87
5.5.2 Z-Optimization (FI Top measurements with the Infinite M200 PRO only)88
5.5.3 FI Ratio Mode ....................................................................................... 93
5.5.4 Optimal Read........................................................................................ 94
5.6 FP Measurements ............................................................................... 96
5.6.1 Fluorescence Polarization .................................................................... 96
5.6.2 Measurement Blank Range .................................................................. 96
5.6.3 G-Factor Settings ................................................................................. 97
5.6.4 Measurement with an Uncalibrated G-Factor ....................................... 97
5.6.5 Measurement with a Simultaneous G-Factor Calibration ..................... 98
5.6.6 Measurement with a Calibrated G-Factor ............................................. 99
5.6.7 Measurement with a Manual G-Factor ............................................... 100
5.6.8 Calculation of Fluorescence Polarization Parameters ........................ 101
5.7 Optimizing Absorbance Measurements ......................................... 102
5.7.1 Measurement Parameters .................................................................. 102