5. Operating the Instrument
2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 77
Temperature Control
Some assays require an exact operating temperature. The Infinite 200 PRO can
set up a specific temperature within a specific range, provide uniformity across
the plate, and keep the temperature constant above ambient. The main cooling
fans stop ventilation.
Heating up the measurement chamber will take some time. Please check the
temperature control display. If not incubated externally, the microplate should be
left for equilibration before the measurement is started.
Temperature range: 5 °C above ambient to 42 °C.
Kinetic Measurements
i-control allows a plate to be measured repeatedly in equidistant time intervals.
Fluorescence signal may significantly decrease over a longer period of time,
especially when using low volumes. Depending on the amount of evaporation, the
meniscus will shift to a lower position giving rise to slightly out of focus conditions.
Usually, wells in the corner evaporate faster, the next at the edges of the
microplate. When measuring fluorescence, decrease in signal may also result
from photo bleaching.
Microplate Shaking
The Infinite 200 PRO provides two shaking modes: linear and orbital. The
shaking amplitude can be selected from 1 – 6 mm in steps of 0.5 mm. The
frequency is a function of the amplitude. The shaking duration is selectable from
1 – 1000 s.
Multi-labeling
The i-control software provides a basic multi-labeling capability. Up to four sets of
instrument parameters can be edited. The corresponding plate measurements
will be executed in the selected order. For example, when using more than one
fluorescent label, different filter combinations could be selected. A multi-labeling
measurement can be set up by using a plate strip with/without a ‘part of the
plate’-strip and up to 10 measurement strips (absorbance fixed wavelength,
absorbance scanning, fluorescence intensity, fluorescence intensity scanning,
luminescence).
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