5. Operating the Instrument
90 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04
For each selected label, one or two wells of the defined plate range can be used
for the z-position optimization. Select the well(s) and click ‘Scan’ to start the z-
optimization:
The z-positioning option ‘Max S/B Ratio’ requires the measurement of two wells,
one filled with a fluorophore of interest (signal) and one filled with buffer (blank).
Both wells are scanned and the resulting signal and blank curves are shown in
the graph. The z-position may now be set to the maximum signal-to-blank (S/B)
ratio:
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