Waters 515 HPLC - Page 10

x Table of Contents
2 Installing the Waters 515 Pump .......................................................... 2-1
Site requirements ............................................................................................. 2-2
Unpacking ........................................................................................................... 2-3
Making electrical connections ....................................................................... 2-4
Replacing the power supply fuses................................................................... 2-4
Connecting the power supply .......................................................................... 2-5
Connecting for remote operation..................................................................... 2-6
Making fluidic connections ............................................................................ 2-6
Connecting the eluent supply.......................................................................... 2-6
Connecting the pump outlet............................................................................ 2-8
Connecting for high-pressure gradient operation........................................ 2-11
3 Using the Waters 515 Pump ................................................................. 3-1
Powering on the pump .................................................................................... 3-2
Power-on diagnostics ....................................................................................... 3-2
Diagnostics failure ........................................................................................... 3-3
Keypad and display overview ........................................................................ 3-3
Keypad.............................................................................................................. 3-4
LCD display ..................................................................................................... 3-5
Editing or entering parameters in local or remote modes........................... 3-12
Preparing the 515 pump ................................................................................ 3-13
Priming the pump.......................................................................................... 3-15
Purging the system........................................................................................ 3-17
Equilibrating the system............................................................................... 3-18
Operating the pump ....................................................................................... 3-21
Stand-alone pump operation......................................................................... 3-21
Remote pump operation ................................................................................ 3-22
Changing parameters during a run ............................................................ 3-24
Adjusting for eluent compressibility .......................................................... 3-24
Required materials ........................................................................................ 3-25
Procedure........................................................................................................ 3-25