Calibrating the pump 4-5
13. If the pressure decay for either pump head is greater than 0.15, you may
have dirty check valves (see “Cleaning and replacing check valves” on
page 4-18). Remove and clean the inlet and outlet check valves. If the
pump problem persists, replace the check valves.
14. When you finish, remove the compression plugs from the draw-off valve
and pump outlet, reconnect the right pump head outlet to the draw-off
valve, then reconnect the pump to the HPLC system.
Calibrating the pump
The Calibrate menu selections let you set or calibrate the following pump
parameters:
• Zero Pressure
•Head size
• Flow rate
• Strokes on seals
These parameters are set at the factory and do not require regular
adjustment. Adjustment is necessary if you have recently replaced a pump
component or performed a maintenance procedure that affects pump
performance (cleaning or replacing pump seals, for example). See the
procedures that follow for more details.
Setting zero pressure
The following procedure describes how to set the Zero Pressure parameter for
the 515 Pump. Set the Zero Pressure parameter if either of the following
conditions occurs:
• You notice significant pressure (or any negative pressure) displayed on
the pump at zero flow rate with no backpressure.
• You were referred to this procedure from “Troubleshooting” on
page 4-26.
To set the zero pressure for the pump:
1. To eliminate backpressure, disconnect the pump outlet from any HPLC
system components.
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