3-18 Using the Waters 515 Pump
Equilibrating the system
Equilibrate your HPLC system when one or more of the following conditions
occur:
• You start your system for the first time.
• After you change eluents or add fresh eluent.
• After you change columns.
• After you leave the system idle for some length of time.
Follow the equilibration procedure (described below) that is appropriate for
your HPLC system:
• If your pump is running in the Local mode (not remotely controlled by a
data system or external controller), follow the procedure in
“Equilibrating for Stand-Alone Operation.”
• If your pump is running in the Rem mode (controlled by a data system or
external controller), follow the procedure in “Equilibrating for remote
operation” on page 3-19.
Before equilibrating
• Make sure the eluent is filtered and degassed.
• Prime the pump with fresh eluent as described in “Priming the pump”
on page 3-15.
• Allow the eluent and the system to reach proper temperature before
pump operation.
Equilibrating for stand-alone operation
To equilibrate for stand-alone operation:
1. Press the Up, Down, or Menu key to display the Flow parameter.
2. Set the pump Flow parameter to the desired rate.
a. Press the Edit/Enter key until the blinking cursor is positioned over
the first digit that you need to edit.
b. Use the Up or Down key to increase or decrease the value of the
digit.
c. If necessary, repeat steps a and b for the other digits until the
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