Cleaning and replacing seals and plungers 4-13
9. Reset the Strokes on Seals parameter as described in “Resetting strokes
on seals” on page 4-8.
10. Perform the following steps:
a. Reconnect the inlet and outlet tubing to the pump head.
b. Reconnect the eluent line to the pump inlet manifold.
c. Remove the fitting plug from the pump outlet, then reposition the
eluent reservoir.
d. Prime the pump as described in “Priming the pump” on page 3-15. If
you notice leaks, verify pump head and plunger seal installation.
e. Recalibrate the pump flow rate as described in “Calibrating the flow
rate” on page 4-7.
Cleaning and replacing the plungers
The plungers are sapphire rods that require careful handling. Although
cleaning the plungers is not difficult, it is important to follow these
instructions carefully to avoid damaging the plungers. Assemble all materials
and read the procedure thoroughly before you begin.
Required materials
• 5/16-inch open-end wrench (Startup Kit)
• 5/32-inch Allen wrench (Startup Kit)
• 9/64-inch Allen wrench (Startup Kit)
• Snap-ring pliers
• Plunger insertion tool (part number WAT011042)
•Sonicator
•HPLC-grade water
• HPLC-grade methanol
• Replacement plunger
• Replacement plunger seals
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