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Waters 515 HPLC - Cleaning and replacing seals and plungers

Waters 515 HPLC
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Cleaning and replacing seals and plungers 4-9
currently installed plunger seals. Perform the procedure only after you replace
plunger seals.
To reset the strokes on seals parameter:
1. Press the Up, Down, or Menu key until the Calibrate menu appears.
2. Press Edit/Enter twice to access the submenu selections.
3. Press Menu to scroll to the Strokes on Seals parameter. The screen
displays the number of plunger strokes for the current seals (assuming
this parameter was reset when the seals were last replaced).
4. Press Edit/Enter to access the Strokes on Seals submenu.
5. When prompted, press Edit/Enter to reset the parameter.
6. Press Menu until you are prompted to exit.
7. Press Edit/Enter to exit the Calibrate menu.
Cleaning and replacing seals and plungers
The plungers in the Waters 515 pump are ultra-smooth, chemically inert
sapphire rods. Salt crystals that precipitate out from the eluent can form on
the plunger and cause wear on the plunger seals and on the plunger itself. The
result is a slow leak and a very slight cyclic pressure fluctuation, with a
possible increase in retention time. In addition, you may need to replace seals,
plungers, or check valves if you were referred to this section from
“Troubleshooting” on page 4-26.
For continued high performance of the 515 Pump:
Replace the plunger seals twice yearly, or as needed.
Clean and inspect the plunger every 6 months (or sooner if using
abrasive eluents).
Replace the plunger yearly, or as needed.
Replacing the plunger seals
Plunger seals can become worn if the plunger becomes dirty or scratched. To
check the number of strokes for the current set of seals, look at the value for
the Strokes on Seals parameter (see “Resetting strokes on seals” on page 4-8

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