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Waters 515 HPLC - Page 68

Waters 515 HPLC
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4-10 Maintaining and Troubleshooting the Waters 515 Pump
for details). Each time you replace the plunger seals, you need to reset the
Strokes on Seals parameter.
Required materials
5/16-inch open-end wrench (Startup Kit)
5/32-inch Allen wrench (Startup Kit)
Priming syringe (Startup Kit)
Seal insertion tool (part number WAT076765)
Fitting plug (Startup Kit)
Replacement seal kit (see Appendix C, Accessories and Spare Parts, for
part numbers)
Methanol
Removing the pump head
To remove the pump head:
1. Purge the pump with methanol as described in “Purging the system” on
page 3-17. If methanol is not miscible with your current eluent, use an
intermediate eluent.
2. Disconnect the eluent line from the inlet on the pump. Disconnect the
outlet tubing from the pump and install a fitting plug in the outlet.
Tighten the plug with the 5/16-inch wrench.
3. Insert the priming syringe into the luer fitting at the center of the
draw-off valve handle, then turn the handle counterclockwise about
1/2-turn to open the valve.
4. Use the syringe to withdraw all methanol.
5. Set the Flow parameter to 0.3 mL/min, then press Run/Stop to run the
pump.
6. When the indicator rod fully retracts into the pump head, press
Run/Stop again to turn off the pump. This ensures that the weight of the
pump head does not rest on the plunger while you remove the head.
Caution: Before you continue with this procedure, lower the eluent
reservoir to eliminate gravity flow of eluent.

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