Waters 515 HPLC - Operating the pump

Operating the pump 3-21
Operating the pump
This section describes how to operate the 515 pump in either a stand-alone or
remote configuration.
Stand-alone pump operation
Follow the steps in this procedure if your HPLC system is set up in an
isocratic configuration where you manually control the operation of the 515
pump from the pump keypad.
Before operation
Before you operate the pump in a stand-alone configuration, make sure:
• You have already completed any required priming, purging, and
equilibrating as described in “Preparing the 515 pump” on page 3-13.
• Eluent reservoirs are filled with filtered, degassed eluent.
Operating
To operate the 515 pump in a stand-alone configuration:
1. Press the Up, Down, or Menu key to display the Flow parameter. If the
setting is correct, go to step 3.
2. To change the Flow parameter:
a. Press the Edit/Enter key until the blinking cursor is positioned over
the first digit that you need to edit.
b. Use the Up or Down key to increase or decrease the value of the
digit.
c. If necessary, repeat steps a and b for the other digits until the
parameter value is correct.
d. Press Menu to store the parameter.
Warning: To avoid chemical hazards, observe safe laboratory practices
when you handle eluents. See the Material Safety Data Sheets for the
eluents you use. For more information on eluent properties, see
Appendix D, Eluent Considerations.