Waters 515 HPLC - Page 52

3-20 Using the Waters 515 Pump
To equilibrate for remote operation:
1. Press the Up, Down, or Menu key to advance past the Flow, HPL, and
LPL parameters.These pump parameters are set from the data system
or external controller.
• If the pump is connected to a Waters Millennium
32
system through a
PCM, use the Equilibrate function and an appropriate method set,
or use the Monitor Baseline control button and an instrument
method to handle equilibration. See the Millennium
32
documentation.
• If the pump is connected to a Waters ExpertEase system through a
PCM, use the Monitor Baseline selection and a specified method to
handle equilibration. See the ExpertEase documentation.
• If the pump is connected to a Waters 680 Automated Gradient
Controller (AGC), set the pump parameters at the controller keypad
(see the Waters Model 680 Automated Gradient Controller
Operator’s Manual).
Tip: When the 515 pump is connected to a Waters 680 Automated
Gradient Controller, make sure the pump HPL parameter is set to 6000
psi (41370 kPa, 401 bars) and the pump Comp parameter is set to 0
(zero). In addition, you must set the flow rate, high-pressure limit, and
low-pressure limit at the controller.
2. Press Menu until the Mode parameter appears. If required, press the
Edit/Enter key, then the Up or Down key to set the parameter to Rem.
Press Menu to store the parameter.
3. Press Run/Stop. This places the pump in the Run – Rem mode, and the
Flow area of the display reads No Flow. The pump is ready to receive a
remote run signal to start the equilibration.
4. Start the equilibration from the data system or from the external
controller. The pump starts and the Flow area of the pump display reads
Flow.
Caution: Be sure that the entered values for flow rate,
high-pressure limit, and low-pressure limit are appropriate for
your column. Some columns require a flow ramp up. See the Care
and Use Manual for your column.