Waters 600e User Manual

Waters 600E
Multisolvent Delivery System
User’s Guide
34 Maple Stre
et
Milford, MA 0175
7
WAT174-02TP, Revision
3
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www.Chromatography.co

Waters 600e Specifications

General

Model	600E
Weight	Not specified in available documentation
Flow Rate	0.001 to 10 mL/min
Pressure Range	0 to 6000 psi
Solvent Compatibility	Compatible with a wide range of solvents
Maximum Flow Rate	10.0 mL/min
Voltage	100-240 VAC

Summary

Chapter 1 Introduction

1.1 Waters 600E System Overview

Provides a general overview of the Waters 600E HPLC system's features.

1.2 Waters 600E System Modules

1.3 PowerLine Controller Features

Details the capabilities and description of the PowerLine Controller configuration.

1.4 Gradient Controller Features

Outlines the functionalities offered by the Gradient Controller configuration.

Chapter 2 Basic Operation

2.1 Powering Up the System

Provides instructions for powering up the Waters 600E system.

2.2 Running Diagnostics

An overview of the diagnostic routines available for the 600E system.

2.3 Using the Controller Front Panel

Explains the components and usage of the controller's front panel.

2.4 Comparing Controller Types

Helps determine the best controller configuration (PowerLine vs. Gradient).

2.5 Powering Down the System

Provides procedures for safely powering down the Waters 600E system.

Chapter 3 Preparing Your 600E System for Operation

3.1 Sparging Eluents

Details the procedure for using the helium sparge feature to degas reservoirs.

3.2 Priming the Pump

Explains the process of priming the pump for proper operation.

3.3 Daily Routine Operation

Provides information for daily system preparation and operation.

3.4 Flushing the System

Ensures all eluent is replaced with new eluent before running samples.

3.5 Equilibrating the System

Sets initial parameters to equilibrate the system for injections.

3.6 Changing Eluents

Outlines recommended procedures for changing eluents in the system.

Chapter 4 Setting PowerLine Controller Operating Parameters

4.1 Setting Up the 600 as a PowerLine Controller

Describes configuring the Waters 600 Controller as a PowerLine Controller.

4.2 Verifying that PowerLine Devices Are Active

4.3 Setting Up the 600E Pump

4.4 Setting Up the Autoinjector

4.5 Setting Up Dissolution Control

4.6 Setting Up the Detectors

Chapter 5 Running PowerLine in Direct Control Mode

Direct Control

5.1 Setting Run Conditions

Procedures for setting pump, event, autoinjector, and detector parameters.

5.2 Performing a Direct Control Run

Details the steps for performing a run using the Direct Control screen.

5.3 Running a Method from the Direct Control Screen

Chapter 6 Creating PowerLine Methods

6.1 Creating a Gradient Table

6.2 Creating an Event Table

6.3 Creating a Detector Table

6.4 Creating a Method Table

Chapter 7 Running PowerLine Methods

7.1 Using an Injector

7.2 Setting Initial Operating Parameters

7.3 Starting a Run

Covers accessing the Operate Methods screen and running a method.

7.4 Modifying Conditions While Running

Chapter 8 Setting Gradient Controller Operating Parameters

When to Use Gradient Controller Mode

8.1 Setting Up the 600 as a Gradient Controller

8.2 Setting Up the 600E Pump

Chapter 9 Running Gradient Controller in Isocratic Mode

Isocratic Control

9.1 Setting Run Conditions

Procedures for setting run conditions in Isocratic mode.

9.2 Performing an Isocratic Run

Steps for making a run by performing procedures on the Isocratic screen.

Chapter 10 Creating Gradient Controller Time-Based Tables

10.1 Creating a Gradient Table

10.2 Creating an Event Table

Chapter 11 Running Gradients

11.1 Using an Injector

11.2 Setting Initial Operating Parameters

11.3 Starting a Run

Covers accessing the Operate Gradient screen and running a gradient.

11.4 Modifying Conditions While Running

Appendix A Specifications

Appendix B Eluent Considerations

B.1 Eluent Compatibility with the 600E System

B.2 Clean Eluent

B.3 Eluent Miscibility

B.4 Eluent Physical Properties

B.6 Eluent Degassing

Appendix C Analysis Plan

Analysis Plan and File Sheet