Changing Eluents 41
3.6 Changing Eluents
This section outlines the recommended procedures for changing eluents in the Waters
600E System. The procedures include:
• Adding fresh eluent
• Changing between incompatible eluents
Eluent Compatibility
All eluents are compatible with the 600E system. However, long-term static exposure to
halide salts (for example, fluoride, bromide, chloride, and iodide) will cause pitting and
corrosion of stainless steel parts. Flush the system thoroughly with water if the pump will
be idle for more than two days. See Section 3.4, Flushing the System.
3.6.1 Adding Fresh Eluent
Before You Begin
This section assumes that you have already performed the procedures in the preceding
sections:
• Section 3.1, Sparging Eluents
• Section 3.2, Priming the Pump
• Section 3.3, Daily Routine Operation
• Section 3.4, Flushing the System
• Section 3.5, Equilibrating the System
Considerations
Before adding eluent to your system:
• Always follow safe laboratory practices when working with eluents. See the
Material Safety Data Sheets for the eluents in use.
STOP
Attention: Never change directly between immiscible eluents or between
buffered solutions and organic eluents. Immiscible eluents form emulsions in
the flow path. Buffered solutions and organic eluents in combination may
result in salt precipitation in the gradient proportioning valves, pump heads,
check valves, or other parts of the system.
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