Performing a Direct Control Run 97
5.2.3 Equilibrating the System
When the system status displays Initial, the system is running with the conditions
displayed on the Direct Control screen and with the downloaded PowerLine module
operating parameters. The system runs at these conditions until you equilibrate the system.
For more details, see Section 3.5, Equilibrating the System.
To equilibrate your system, allow at least 5 to 6 column volumes of eluent (as a minimum,
10 is recommended) to pass through your system flow path. When you have equilibrated
your system, continue with Section 5.2.4, Starting a Run.
5.2.4 Starting a Run
Note: The 600 controller examines the status of the 490E detector before initiating a run.
If the detector is in the standby state, the controller automatically turns on the lamp.
Using the Rheodyne 7725i Manual Injector
To begin a run with the Rheodyne 7725i manual injector:
1. Load the injector loop with sample. see Section 7.1.1, Using the Rheodyne 7725i
Manual Injector.
2. Turn the injector handle to the Inject position to make the injection. The injector
sends an Inject signal to the controller.
Using a PowerLine Autosampler
To begin a run with a PowerLine autosampler (717plus):
1. Load the sample vials into the autosampler.
2. Press the Start Run screen key. The 600 controller automatically starts the
autosampler. The system status changes from Initial to Inject Wait.
3. The autosampler draws the sample and makes the injection. The controller receives
the Inject signal through the IEEE-488 bus.
Using a Non-PowerLine Autoinjector
Note: If you are using an autoinjector, see the documentation provided with that product
for information on sample loading.
To begin a run with an external non-PowerLine autoinjector:
1. Load the sample vials into the autoinjector.
2. Program and start the autoinjector. See the autoinjector documentation.
To Next Page
Loading...
