42 Preparing Your 600E System for Operation
• Always wear safety glasses when working with eluents.
• If your column is not compatible with an eluent, disconnect the column and replace
the column in line with a union.
• If organic eluents and aqueous buffers are used together, make certain that salt does
not precipitate at any composition. Salts damage pump heads and valves.
Adding Fresh Eluent
Procedure
1. Filter and sparge the fresh eluent. Confirm that the vent lines are properly routed to
an exhaust hood. See Appendix B, Eluent Considerations, and Section 3.1, Sparging
Eluents, for more information.
2. Prime and flush the system with new eluent using the procedures described in
Section 3.2, Priming the Pump, and Section 3.4, Flushing the System.
3.6.2 Changing Between Incompatible Eluents
This section describes the procedures for changing between incompatible eluents,
including:
• Flushing the system with an intermediate eluent (described below)
• Adding the final eluent to the system (see Section 3.6.1, Adding Fresh Eluent)
You must perform the above-mentioned procedures whenever you change between the
following types of incompatible eluents:
• Organic eluents and buffered solutions
• Immiscible eluents
Considerations
Before you begin, take into account the following considerations:
• If organic eluents and aqueous buffers are used together, be careful not to precipitate
salt in the pump heads and check valves during eluent changes.
STOP
Attention: Before priming and flushing, place the reference valve in the
open position, or disconnect the pump outlet tube from the column inlet tube
to take the column out of the flow path. If you disconnect the pump outlet
tube, be sure to place it in a waste beaker.
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