166 Running Gradient Controller in Isocratic Mode
• Prepared your system as outlined in Chapter 3, Preparing Your 600E System for
Operation
• Set operating parameters as described in Chapter 8, Setting Gradient Controller
Operating Parameters
9.1 Setting Run Conditions
Before you make a run with the PowerLine controller in the Isocratic mode, you must first
set the run conditions. This section describes the procedures for:
• Setting pump parameters
• Setting external event switches
Run Requirements
To perform an isocratic run in the isocratic mode, you must:
• Set the run conditions (flow rate, eluent composition, sparge rate, heater module
temperature, and external switch output settings)
• Set the run time
• Equilibrate the system
• Start the run
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