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Waters 600e - Changing Parameters; Holding Conditions Constant; Stopping Flow

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144 Running PowerLine Methods
7.4.1 Changing Parameters
You can change the following parameters during a run:
•Sparge rate
Column heater temperature
Event switch status (On/Off)
Operating parameters for any of the detectors
Note: You can change parameters only if the keyboard is unlocked.
To change a parameter on the Operate Methods screen:
1. Move the cursor to the appropriate parameter field on the Operate Methods screen
(see Figure 7-4).
2. Type the new value. Press Enter.
When you manually enter a change, the new value remains in effect until the next timed
program change.
7.4.2 Holding Conditions Constant
The Hold function keeps the eluent flow rate and composition constant during a run,
which is a useful feature in methods development. The system suspends the run time and
external events program while this function is in effect.
To suspend system operation at the current conditions:
1. Press the Hold screen key on the Operate Methods screen. The Hold key acts as a
toggle, and changes to Resume when you press it.
When you press Hold both the gradient clock and the Hold clocks stop.
2. To continue system operation from the point at which you suspended it, press the
Resume screen key. The key label changes back to Hold, and the gradient and
run-time clocks continue from where they stopped.
7.4.3 Stopping Flow
You generally use the Stop Flow screen key at the end of an analysis. However, you can
also use it to temporarily shut down your pump (for example, to adjust a fraction collector)
or to function as an emergency shutdown.
The Stop Flow function also suspends the method table, but does not stop the run-time
clock. The current event and detector tables continue to execute. However, pressing Stop

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