Flushing the System 37
5. Fully insert the syringe needle into the injector port.
6. Gently discharge the contents of the syringe to completely fill the sample loop and
expel any old or intermediate eluent from the injector vent tube. This step replaces
the eluent in the sample loop with fresh eluent.
7. Leave the syringe in position and turn the injector handle to the Inject position.
8. Remove the syringe.
9. Allow the eluent to flow for several minutes at 1.0 mL/min.
3.4.3 Purging the Autoinjector
Perform the following steps to purge a Waters autoinjector. If you are using a non-Waters
autoinjector, see the documentation provided with the autoinjector for the appropriate
steps to flush the autoinjector flow path.
1. Move the cursor to the Vial field on the Direct Control screen (see Figure 3-2).
2. Type 103 and press Enter. (103 is the special vial code that places the autoinjector in
its purge mode.)
The default run time is 4.5 minutes. Enter a longer time if 4.5 minutes is too short
for your application.
3. If necessary, continue to one of the following sections:
• Section 3.4.4, Reconnecting the Column After Flushing the System, if the
column is not in the flow path
• Section 3.5, Equilibrating the System, if the column is in the flow path
3.4.4 Reconnecting the Column After Flushing the System
Perform the following procedure if the column has been replaced with a union, and if the
eluent inlet tubing, gradient proportioning valve, pump, injector, and detector flow cells
are filled with new eluent.
1. Press the Stop Flow screen key to stop the pump.
2. Remove the union and reinstall the column. See the column Care and Use Manual
for column operating guidelines.
3. Continue with Section 3.5, Equilibrating the System, to prepare the system for
samples.
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