Eluent Miscibility 213
• When using a high-ionic-strength buffer, flush all fluid pathways with distilled
water before shutting down the system. Leave distilled water in the system (flush
with 10% methanol in water for shutdowns scheduled to be more than one day). See
Section 2.5, Powering Down the System.
B.3.1 Eluent Properties Table
Table B-1 lists the physical properties of a series of eluents. Use this table in conjunction
with Section B.3.2, Using Miscibility Numbers (M-Numbers).
Table B-1 Physical Properties of Eluents
Polarity
Index
Eluent
Viscosity [η] CP,
20 °C
Boiling Point °C
(1 atm)
Miscibility
Number (M)
0.0 Hexane 0.313 68.7 29
0.0 Cyclohexane 0.98 80.7 28
0.3 n-Decane 0.92 174.1 29
0.4 Octane 0.50 99.2 29
1.7 Butyl ether 0.70 142.2 26
1.8 Triethylamine 0.38 89.5 26
2.2 i-Propyl ether 0.33 68.3 26
2.3 Toluene 0.59 101.6 23
2.4 p-Xylene 0.70 138.0 24
3.0 Benzene 0.65 80.1 21
3.3 Benzyl ether 5.33 288.3 26
3.4 Methylene chloride 0.44 39.8 20
3.4 Chloroform 0.57 61.2 19
3.7 Ethylene chloride 0.79 83.5 20
3.9 i-Butyl alcohol 3.00 117.7 15
4.2 Tetrahydrofuran 0.55 66.0 17
4.3 Ethyl acetate 0.47 77.1 19
4.3 1-Propanol 2.30 97.2 15
4.3 2-Propanol 2.35 117.7 15
4.4 Methyl acetate 0.45 56.3 15, 17
4.5 Methyl ethyl ketone 0.43 80.0 17
To Next Page
Loading...
