Setting Run Conditions 93
Inj Vol
Specifies the size of the sample you wish to inject.
To select the injection volume:
1. Move the cursor to the Inj Vol field.
2. Type the appropriate sample value to be injected.
3. Press Enter.
Valid entries are 0 to 200 mL with the standard sample loop installed, or 0 to 2000 mL
with the optional sample loop installed.
5.1.4 Setting Detector Parameters
Use the Direct Control screen (see Figure 5-2) to:
• Set attenuation and wavelength values for Waters PowerLine UV/Vis detectors
(Waters 486 and 490E)
• Turn the 486 detector lamp On/Off
• Set sensitivity for a PowerLine refractive index (RI) detector (Waters 410) or a
conductivity detector (Waters 431/432)
Setting Attenuation and Wavelength for UV/Vis Detectors
To set the attenuation and wavelength for an absorbance detector:
1. Move the cursor to the AUFS field for a UV/Vis detector (Detector #1).
2. Type an AUFS value between 0.001 and 2.00 (AU) for the detector. Press Enter.
3. At the λ field, type an operating UV/Vis wavelength between 190 and 600 (nm).
Press Enter.
4. Repeat steps 1 through 3 if using a second UV/Vis detector.
Note: Ensure that the 600 controller recognizes the Waters 486 detector on the IEEE-488
bus. See Section 4.2, Verifying that PowerLine Devices Are Active.
Controlling the 486 Detector Lamp
Note: Waters recommends that in order to conserve lamp life, do not turn the lamp off for
less than 4 hours.
To turn On/Off the 486 detector lamp:
1. Press the More screen key.
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