38 Preparing Your 600E System for Operation
3.5 Equilibrating the System
To equilibrate the system, set the following initial parameters on the Direct Control screen
(see Figure 3-2):
•Flow rate
• Eluent composition
•Sparge rate
• AUFS
• Sensitivity
Note: When using the Millennium Chromatography Manager or the ExpertEase 845/860
Data System, see Section 8.1.2, Communicating with a Data System, for information on
configuring the data system. The data system will help determine when the baseline is
stable and the system is ready for injections.
When to Equilibrate
Equilibrate the system:
• When starting up your system for the first time (after eluents are sparged)
• After changing eluents
• After changing eluent composition
• After changing columns
• When preparing to make a gradient separation
• After an idle period
Good Operating Practices
When you equilibrate your system:
• Maintain sufficient eluent in the mobile phase reservoirs to ensure that the reservoirs
do not run dry while operating. Running the pump without eluent adversely affects
seal life.
• Equilibrate both the eluent and the system at ambient temperature before pump
operation. Cold eluents that flow through a system at room temperature outgas as
they warm up.
• If you are operating in a cold room, equilibrate the system at the reduced
temperature.
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