Equilibrating the System 39
Procedure
To equilibrate the system:
1. Press the Direct function key to access the Direct Control screen (see Figure 3-2).
Sparging
2. If desired, set the sparging conditions as follows:
a. Move the cursor to the Sparge field.
b. Type in the maintenance rate for your eluents. Press Enter. All reservoirs that are
enabled for sparging on the Pump Setup screen sparge at that rate.
c. The actual sparge rate is dependent on your analytical requirements. An initial
high sparge flow rate that reduces to a low maintenance rate, is recommended.
For guidelines and information about sparging, see Section 3.1, Sparging
Eluents, and Appendix B, Eluent Considerations.
Setting Eluent Composition
3. To set the initial eluent composition:
a. Move the cursor to the New %A field.
b. Type the % composition for the mobile phase (0 to 100, no decimals, in 1%
increments) from reservoir A. Press Enter.
c. Repeat the previous two actions for the remaining three reservoirs. The sum of
the four % composition entries must equal 100.
d. The system implements the changes when you press Enter after typing the value
for eluent D. The Current column displays the new composition.
Setting Flow Rate
4. Set the initial flow rate as follows.
Note: Some columns require a flow ramp up. See the column Care and Use Manual
for guidelines.
a. Move the cursor to the Flow field.
STOP
Attention: Be sure that the values for the maximum and minimum operating
pressures programmed on the Pump Setup screen are appropriate for your
column. See the column Care and Use Manual for your column. For
information on setting the minimum and maximum pump pressure values,
see Section 4.3.3, Exiting the Pump Setup Screen (for PowerLine Controller
operation), or Section 8.2.3, Exiting the Pump Setup Screen (for Gradient
Controller operation).
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