40 Preparing Your 600E System for Operation
b. Type in the desired flow rate value (0 to 20 or 45 mL/min). Press Enter.
The pump immediately begins operating at the new flow and eluent composition
conditions.
Setting PowerLine Detector Parameters
5. Set the PowerLine detector parameters as necessary by moving the cursor to the
appropriate detector column.
For a PowerLine UV/Vis absorbance detector:
• At the AUFS field, type the required absorbance units full scale (AUFS) value.
Press Enter. Valid values are 0.001 to 2.00 AU.
• At the l field, type the required detector operating wavelength. Press Enter. Valid
entries are 190 to 600 nm.
For a PowerLine refractive index detector (Waters 410), at the Sens field, type the
required sensitivity. Press Enter. Valid values are 1, 2, 4, 8, 16, 32, 64, 128, 256, 512,
and 1024.
For a PowerLine conductivity detector (Waters 431/432), at the Sens field, type the
required sensitivity in microSiemens (µS). Press Enter. Valid values are 0.1, 0.05,
0.02, 0.01, 0.005, 0.002, 0.001, and 0.0005.
6. Allow a minimum of five to six column volumes to pass through the system before
checking the stability of the baseline.
7. Observe the Press (pressure) value on the Direct Control screen. The pressure
fluctuations should be within 5 to 10 percent of the average pressure value.
8. If the pressure fluctuates greatly, or if every other pump stroke produces a
significant pressure drop, repeat the procedures in Section 3.2, Priming the Pump
and Section 3.4, Flushing the System, to purge the pump of air. If this does not
correct the problem, see the Waters 600E Multisolvent Delivery System Installation
and Maintenance Guide, Chapter 5, Troubleshooting, for additional help.
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