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Waters 600 User Manual

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Waters 600E
Multisolvent Delivery System
Users Guide
34 Maple Stre
et
Milford, MA 0175
7
WAT174-02TP, Revision
3
View More Waters Manuals at
www.Chromatography.co

Table of Contents

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Waters 600 Specifications

General IconGeneral
Model600
Number of Solvents4
Gradient Composition Accuracy±0.5%
Power Requirements100-240 VAC, 50/60 Hz
Flow Rate0.01 to 10.0 mL/min
Pressure Range0-6000 psi
Flow Accuracy±1%

Summary

Commonly Used Symbols

Caution, Risk of Electric Shock (High Voltage)

Warns about potential electrical shock hazards, especially with high voltage.

Chapter 3 Preparing Your 600E System for Operation

Preparing the System in PowerLine and Gradient Mode

Procedures for system preparation in both PowerLine and Gradient modes.

3.1 Sparging Eluents

Instructions for using the system's helium sparge feature for eluents.

3.2 Priming the Pump

Procedures for priming the pump to ensure proper operation.

Chapter 1 Introduction

1.1 Waters 600E System Overview

Provides an overview of the Waters 600E system's features.

1.2 Waters 600E System Modules

Discusses the major modules of the Waters 600E system.

1.3 PowerLine Controller Features

Details the capabilities and description of the PowerLine Controller.

1.4 Gradient Controller Features

Outlines the features and capabilities of the Gradient Controller.

Chapter 2 Basic Operation

2.1 Powering Up the System

Instructions for powering up the Waters 600E system.

2.3 Using the Controller Front Panel

Explanation of the controller front panel layout and key functions.

2.5 Powering Down the System

Procedures and precautions for powering down the system.

Chapter 4 Setting PowerLine Controller Operating Parameters

4.1 Setting Up the 600 as a PowerLine Controller

Procedures to configure the Waters 600 Controller as a PowerLine Controller.

4.1.1 Configuring the Controller Type

Describes how to verify or change the Waters 600 Controller type.

4.3 Setting Up the 600E Pump

Describes how to access and use the Pump Setup screen.

4.3.2 Setting Pump Setup Parameters

Describes pump parameters and procedures for setting them.

4.6.2 Setting Detector Setup Parameters

Describes detector setup parameters and procedures for setting them.

Chapter 5 Running PowerLine in Direct Control Mode

5.1 Setting Run Conditions

Procedures for setting run conditions before making a run.

5.2 Performing a Direct Control Run

Procedures for making a run using the Direct Control screen.

5.2.4 Starting a Run

Procedures for starting a run with different injector types.

Chapter 6 Creating PowerLine Methods

6.1 Creating a Gradient Table

Information needed to create the first element of a method table, the gradient table.

6.1.2 Programming the Gradient Table

Provides instructions for entering and editing gradient table parameters.

6.1.3 Entering Parameter Values in the Gradient Table

Steps to enter parameter values into the Program Gradient table.

6.2 Creating an Event Table

Information to create the second element of a method table, the event table.

6.2.2 Programming the Event Table

Illustrates steps for using the Program Event screen to create an event table.

6.2.3 Entering Parameter Values in the Event Table

Steps to enter parameter values into the Program Event table.

6.3 Creating a Detector Table

Information to create the third and last component of a method table, the detector table.

6.3.2 Programming the Detector Table

Illustrates steps for using the Detector Table screen to create a table.

6.3.3 Entering Parameter Values in the Detector Table

Steps to enter parameter values into the Detector Table.

6.4 Creating a Method Table

Links gradient, event, and detector tables into a method table.

6.4.2 Programming the Method Table

Illustrates steps for using the Program Methods screen to create a Method Table.

6.4.3 Entering Parameter Values in the Method Table

Steps to enter parameter values into the Program Methods table.

Chapter 7 Running PowerLine Methods

7.2 Setting Initial Operating Parameters

How to set initial parameters for running method tables.

7.3 Starting a Run

Procedures for starting a run after setting initial operating conditions.

7.3.3 Running a Method

How to run a method using different injector types.

Chapter 8 Setting Gradient Controller Operating Parameters

8.1 Setting Up the 600 as a Gradient Controller

Procedures to configure the Waters 600 Controller as a Gradient Controller.

8.1.1 Configuring the Controller Type

Describes how to verify or change the Waters 600 Controller type.

8.2 Setting Up the 600E Pump

Describes how to access and use the Pump Setup screen in Gradient Control mode.

8.2.2 Setting Pump Setup Parameters

Describes pump parameters and procedures for setting each parameter.

Chapter 9 Running Gradient Controller in Isocratic Mode

9.1 Setting Run Conditions

Procedures for setting run conditions in Isocratic mode.

9.2 Performing an Isocratic Run

Procedures to make a run using the Isocratic screen.

9.2.2 Starting a Run

Procedures for starting a run with different injector types.

Chapter 10 Creating Gradient Controller Time-Based Tables

10.1 Creating a Gradient Table

How to define and document information for the gradient table.

10.1.2 Programming the Gradient Table

Illustrates steps for using the Program Gradient screen to create a gradient table.

10.1.3 Entering Parameter Values into the Gradient Table

Steps to enter parameter values into the Program Gradient table.

10.2 Creating an Event Table

Information to define and document information for the event table.

10.2.2 Programming the Event Table

Illustrates steps for using the Program Event screen to create an event table.

10.2.3 Entering Parameter Values into the Event Table

Steps to enter parameter values into the Program Event table.

Chapter 11 Running Gradients

11.2 Setting Initial Operating Parameters

How to set initial parameters for running gradient and event tables.

11.3 Starting a Run

Procedures for starting a run after setting initial operating conditions.

11.3.2 Running a Gradient

Steps to start the pump at initial conditions and run a gradient.

Appendix B Eluent Considerations

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