34 Preparing Your 600E System for Operation
3. Move the cursor to the Flow field and press 10. Press Enter. This sets the flow rate
to 10 mL/min for eluent reservoir A.
4. Rotate the inlet manifold valve handle fully counterclockwise to the Draw position
(see Figure 3-3). Draw off some eluent, rotate the valve handle to the Inj position
and apply slight pressure to force eluent through the heads. Rotate the valve handle
to the Run position and observe the flow. If flow is not constant repeat this step.
When flow is constant repeat this procedure for other reservoirs that you intend to
use.
5. When the pump has been primed using all applicable reservoirs, press the Stop flow
screen key to stop the pump.
3.3 Daily Routine Operation
This section provides the information you need to prepare the system on a daily basis.
Initial Startup
1. Fill the eluent reservoirs with sufficient eluent for the day’s operation.
2. Power up the system.
Note: Oxygen can dissolve into the eluent overnight. If you are not using fresh
eluent, sparge each filtered eluent reservoir for a minimum of 15 minutes at 100
mL/min before flushing the system (see Section 3.4, Flushing the System) to
replace the older eluent.
Flushing the Pump
To ensure a thorough flushing:
1. Disconnect the pump outlet tube from the column inlet and place the outlet tube in a
waste container.
If thorough flushing is not required, leave the pump outlet tube connected and flush
fresh eluent through the system to waste by opening the reference valve.
2. Press the Direct function key. The Direct Control screen appears.
3. Move the cursor to the Flow Rate field and type a flow rate of 2.00 mL/min.
STOP
Attention: Confirm that the reference valve is open (i.e., injector and column
bypassed) when selecting high flow rates or when making rapid changes in
flow rate. Sudden flow and backpressure variations or high flow rates may
damage installed columns.
To Next Page
Loading...
