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Waters 600 - 9.2 Performing an Isocratic Run; 9.2.1 Equilibrating the System

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Performing an Isocratic Run 171
Connecting External Devices
Before setting any of the event switches, connect the external device to the switch
terminals on the rear panel of the controller. See the Waters 600E Multisolvent Delivery
System Installation and Maintenance Guide for more information on these connections.
Note: S4 defaults to the Off position when a stop-flow condition or a critical fault occurs.
Use this feature by connecting to S4 a device, such as an autosampler, that must be shut
off when an error, such as a high-pressure shutdown or a critical fault, occurs.
Turning On Switches
1. Move the cursor to the appropriate switch field (S1, S2, S3, or S4).
2. Type 1 (On). Press Enter.
Turning Off Switches
1. Move the cursor to the appropriate switch field (S1, S2, S3, or S4).
2. Type 0 (Off). Press Enter.
9.2 Performing an Isocratic Run
Once you have set the run conditions (see Section 9.1, Setting Run Conditions), use the
Isocratic screen (see Figure 9-2) to make a run by performing the procedures in the
following sections:
Equilibrating the system
Starting a run
Changing parameters while running
Stopping the pump
9.2.1 Equilibrating the System
The 600E system operates with the conditions displayed on the Isocratic screen. The
system runs at these conditions until the system is equilibrated. For more details, see
Chapter 3, Preparing Your 600E System for Operation.
To equilibrate your system, allow at least 5 to 6 column volumes of eluent to pass through
the system. When your system is equilibrated, continue with Section 9.2.2, Starting a Run.

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