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Chapter 9
Running Gradient Controller
in Isocratic Mode
Once you have familiarized yourself with the Waters 600E Multisolvent Delivery System
and prepared it according to the procedures in Chapters 1 through 3 and 8, you may make
a run in the isocratic mode.
Isocratic Control
Isocratic operations do not allow time-actuated changes in flow rate or eluent composition
or other time-dependent conditions to occur. To change flow rate or eluent composition, or
to switch output states, you must manually program new values to override the original
parameter settings. The system implements these changes immediately.
Isocratic mode is also useful for preparing the system to make a run. You program
isocratic operation through the Isocratic screen (see Section 9.1, Setting Run Conditions).
By setting parameters on the Isocratic screen, you can do the following without making an
injection:
• Sparge eluents
• Prime the pump (Section 3.2, Priming the Pump)
• Equilibrate the system
• Monitor the baseline (see your detector manual)
• Set external switch parameters
Before You Begin
This chapter assumes that you have:
• Installed your system and all external devices according to the procedures in the
Waters 600E Multisolvent Delivery System Installation and Maintenance Guide
• Familiarized yourself with basic operation and decided upon a controller
configuration (Chapter 2, Basic Operation)