Waters 600 - 3.4 Flushing the System

Flushing the System 35
4. Flush for 2 to 5 minutes.
5. If your system backpressure fluctuates more than 100 psi (7 kg/cm
2
) once the system
has reached the operating flow rate, prime the system again as described in
Section 3.2, Priming the Pump.
6. If the column in the system has been used before, use sufficient eluent to equilibrate
the system. If the column is new, allow eluent to go to waste before connecting the
eluent outlet to the detector. See the associated Column Care and Use Manual for
equilibration instructions.
3.4 Flushing the System
Flushing the system ensures that all eluent in the fluid system is replaced with new eluent
before running samples.
Flushing involves:
• Flushing system fluid lines
• Flushing the manual injector
• Purging the autoinjector
• Connecting the column after flushing
When to Flush
Flush the system when:
• Adding fresh eluent
• Changing eluent
Before You Begin
Before flushing the system:
1. Filter and sparge the fresh eluent. See Appendix B, Eluent Considerations, and
Section 3.1, Sparging Eluents, for more information.
2. Prime the pump. See Section 3.2, Priming the Pump, if this is the first time you are
starting up your system or if you are using miscible eluents. See Section 3.6,
Changing Eluents, to change from one eluent to another.
Caution: Be careful when venting eluents from a pressurized system. Follow good
laboratory practices including the wearing of eye protection.