28 Preparing Your 600E System for Operation
Eluent Considerations
When purging the Waters 600E system:
• Always wear safety glasses when working with eluents.
• Before operation, always check for miscibility with the previous eluent used, or
change eluents.
• If organic and aqueous buffers are used together, ensure that they do not precipitate
salt in the pump heads and valves. Allow proper changeover steps when priming
and recycling programs from final to initial conditions.
• After using halide ions, flush the system thoroughly.
• Maintain sufficient eluent in the reservoirs to ensure that the reservoirs do not run
dry.
3.1 Sparging Eluents
Use the Waters 600E system helium sparge feature to sparge all enabled reservoirs at a
flow rate between 0 and 100 mL/min. At 100 mL/min, the sparge valves are On at all
times. At flow rates less than 100 mL/min, the sparge valves turn on and off periodically
to achieve an average flow rate over time.
Use an initial sparge rate of 100 mL/min for a minimum of 15 minutes to degas the eluents
followed by a reduced rate (for example, 30 to 50 mL/min) to maintain the degassed
condition. See Appendix B, Eluent Considerations, for additional information on sparging.
When to Sparge
Sparge at 100 mL/min for a minimum of 15 minutes when:
• You have not sparged reservoirs for eight hours or more (air can diffuse into the
system)
• You add fresh eluent to the system
Caution: To avoid respiratory problems do not sparge into open air. This action may be
harmful when performed with certain eluents. Use eluent reservoir caps as described in
the Waters 600E Multisolvent Delivery System Installation and Maintenance Guide,
Section 2.2.1, Setting Up the Eluent Reservoirs. Use an exhaust hood to remove fumes
emitted from the reservoirs.
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