134 Running PowerLine Methods
To load the sample into the Rheodyne 7725i:
1. Turn the handle on the manual injector to the Load position (Figure 7-1).
Figure 7-1 Rheodyne 7725i Injector in the Load Position
2. Load a syringe with sample. The loop may be partially filled or overfilled. The
injector is equipped with a 20-µl loop.
3. Fully insert the 22-gauge, 90° point syringe needle into the filler port.
4. Gently discharge the contents of the syringe.
5. Turn the Load/Inject handle to the Inject position.
Caution: Always follow safe laboratory practices when handling eluents. Know the
physical and chemical properties of the eluents. See the Material Safety Data Sheets for
the eluents in use.
Caution: When using sample loops larger than 100-µL, protect yourself from the rapid
ejection of mobile phase from the needle port when you turn the valve from Inject to
Load. A 1-mL loop expands by 20-µL upon decompression from 2900 psi (200 bar).
STOP
Attention: Be sure to use the proper size of syringe needle to avoid damage to the valve.
Proper needle dimensions are 0.028 inches x 2 inches (0.071 cm x 5.08 cm) OD, without
electro taper. The point must be 90° (square).
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