Preparing the 515 pump 3-17
Purging the system
Purging ensures that all eluent in the fluidic path is replaced with new eluent
before you run samples. Purge the system when:
• You start the pump for the first time.
• You change eluent or add fresh eluent.
• You left the system idle for some time.
Before purging
• Make sure the eluent is filtered and degassed.
• Prime the pump with fresh eluent as described in “Priming the pump”
on page 3-15. Once the pump has been primed, install a union in place of
the column.
Tip: When you change between two eluents that are not totally miscible,
perform the steps in this procedure with an intermediate eluent before
purging with the final eluent. See Appendix D, Eluent Considerations for
more information.
Purging
To purge the system:
1. Press the Up, Down, or Menu key until the Purge parameter appears.
1
2. Press Edit/Enter to access the Purge parameter.
3. Press Edit/Enter again to start the Purge. Purge runs the pump at
5 mL/min for 5 minutes. You can stop Purge at any time by pressing
Run/Stop.
4. When the purge is finished, disconnect the union and reconnect the
column.
Caution: To avoid damaging the column, be sure you install a union in
place of the column before you start the pump.
1. As an alternative to running Purge, you can manually set the Flow parameter and run the pump for
the desired time.
To Next Page
Loading...
