4-34 Maintaining and Troubleshooting the Waters 515 Pump
Identifying chromatographic problems
The table below is a guide to troubleshooting and correcting chromatographic
problems.
Identifying chromatographic problems
Symptom Possible cause Corrective action
Erratic retention
times
Air bubble in pump
head
Degas all eluents, prime pump
(see “Priming the pump” on
page 3-15).
Malfunctioning pump
check valves
Clean/replace pump check
valves (see “Cleaning and
replacing check valves” on
page 4-18).
Leaking pump seals Replace pump seals (see
“Cleaning and replacing seals
and plungers” on page 4-9).
Separation chemistry Check eluent, column.
Clogged eluent filters Replace filters.
Increased retention
times
Incorrect flow rate Change flow rate.
Incorrect eluent
composition
Change eluent composition.
Column heater not
turned on
Turn column heater on.
Incorrect eluent Use correct eluent.
Column contaminated Clean/replace column.
Incorrect column Use correct column.
Air bubble in pump
head
Degas all eluents, prime pump
(see“Priming the pump” on
page 3-15).
Clogged eluent filter Replace filter.
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