Troubleshooting 4-35
Reduced retention
times
Incorrect flow rate Change flow rate.
Incorrect eluent
composition
Change composition.
High column
temperature
Reduce column temperature.
Incorrect eluent Use correct eluent.
Column contaminated Clean/replace column.
Incorrect column Use correct column.
Eluent not properly
degassed/sparged
Degas/sparge eluent.
Reproducibility
errors
Eluent not properly
degassed/sparged
Degas/sparge eluent.
Incorrect
chemistry/integration
Check chemistry/integration.
Baseline drift, rapid Column not
equilibrated
Equilibrate column.
Eluent contaminated Use fresh eluent.
Eluent not properly
degassed (rapid or slow
drift)
Degas eluent.
Flow fluctuations (rapid
or slow drift)
Fix pump problems, replace
pump seals, check valves.
Baseline drift, slow Eluent contaminated Use fresh eluent.
Ambient temperature
fluctuations
Stabilize operating
environment temperature
enough to allow full
equilibration.
Identifying chromatographic problems (Continued)
Symptom Possible cause Corrective action
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