3-16 Using the Waters 515 Pump
2. Fully depress the priming syringe plunger to remove all air from the
syringe, then insert the syringe into the luer fitting at the center of the
draw-off valve handle.
Priming the pump
3. Turn the draw-off valve handle counterclockwise about 1/2-turn to open
the valve.
4. Withdraw several milliliters of eluent with the syringe, then close the
valve handle. Expel the contents of the syringe into a suitable waste
container.
5. Press the Run/Stop key to start the pump.
6. Let the pump run for about a minute, then verify normal pump pressure
for the selected eluent and flow rate.
7. If the pump pressure is not correct, repeat steps 2 through 6 as
necessary.
8. Press the Run/Stop key to stop the pump.
Caution: To prevent eluent flow through the pump heads under gravity
pressure, never leave the pump unattended with the draw-off valve in
the open position.
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