D-8 Eluent Considerations
Vacuum sonication
Sonication in combination with a vacuum degasses eluents very quickly. This
technique does not change the composition of mixed eluents appreciably.
Conclusions
With any of the above techniques (except continuous sparging), the eluent
reequilibrates to air saturation in 12 to 24 hours, depending on the eluent.
Degassing by vacuum or sonication or both is often performed for improved
pump performance in multi-pump gradient applications.
Warning: Apply vacuum only to suitable vessels. The brown gallon
bottles in which eluent is shipped are not designed for vacuum
degassing. There is a high risk of implosion if these bottles are used for
vacuum degassing.
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