Making fluidic connections 2-7
• Reservoir containing filtered, degassed eluent
• Stainless steel solvent filter (Startup Kit)
• Plastic tubing cutter (part number WAT031795)
• Razor blade
Connecting eluent tubing to the pump inlet
To connect the eluent tubing to the pump inlet:
1. Measure the length of 1/8-inch PTFE tubing required to connect the
reservoir to the inlet manifold on the pump. Use a reservoir location that
ensures the bottom of the reservoir is about 4 inches (10.2 cm) higher
than the pump inlet. If you are using an in-line degasser, see the
degasser operator’s guide for details on connecting to the reservoir and
pump.
Tip: It may be necessary to substantially increase reservoir height if you
use high-viscosity eluents (18 to 24 inches above the inlet manifold is not
uncommon).
2. Insert the PTFE tubing into the 1/8-inch diameter hole of the tubing
cutter, making sure that the tubing that extends from the metal side of
the cutter is the correct length.
3. Insert the razor blade into the cutter and press down to cut the tubing.
Make sure the cut end is straight and free from burrs.
4. Slide a compression screw over one end of the tubing, followed by a
ferrule with its tapered end facing away from the tubing end, and its
wide end flush with the tubing end, as shown in the figure below.
Reverse ferrule and compression screw assembly
Tubing
Ferrule
(Flush with tubing end)
Compression
screw
Tubing end
(Straight and smooth)
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