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Waters 515 HPLC - Page 29

Waters 515 HPLC
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Making fluidic connections 2-9
Connecting the pump outlet involves:
Cutting the tubing
Attaching a compression fitting to each end of the tubing
Connecting each end of the tubing
Required materials
Two stainless ferrules and standard compression screws (Startup Kit)
1/16-inch OD stainless tubing (Startup Kit)
Circular tubing cutter (part number WAT022384), or knife-edge file
Needlenose pliers (two pairs if cutting tubing with a knife-edge file)
5/16-inch open-end wrench (Startup Kit)
Cutting the tubing to length
To cut the tubing to length:
1. Measure the length of stainless tubing required to connect the pump
outlet to the injector or other instrument.
2. Use a knife-edge file to scribe the circumference of the tubing at the
desired break. Whenever possible, use a circular tubing cutter instead of
the knife-edge file to obtain a clean, square cut.
3. Grasp the tubing on both sides of the scribe mark with cloth-covered
pliers (to prevent marring of the tube surface) and gently work the tube
back and forth until it snaps. Ensure that the break is square and free of
burrs.
Attaching the compression fittings to the tubing ends
To attach the compression fittings to the tubing ends:
1. Remove the black plastic cap from the pump outlet fitting.
2. Slide a compression screw onto one end of the tubing, then slide a ferrule
onto the tubing with the large end of the taper toward the screw, as
shown in the figure below.

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