3-14 Using the Waters 515 Pump
Eluent guidelines
When you prepare a 515 pump for operation, keep in mind the following
eluent guidelines:
• Use methanol to prime a new pump, or one that has been stored for an
extended period.
• Use an intermediate solvent when you change between eluents that are
not miscible.
• Use only chromatography-grade eluents to obtain accurate, reproducible
chromatograms.
• To prevent air from being drawn into the pump, keep the eluent level in
the reservoir at least 4 inches (10 cm) higher than the inlet manifold. Do
not place eluent reservoirs on top of the Waters 515 pump.
• Use only filtered, degassed eluents.
• After use with a buffer, purge the pump with distilled water.
• When possible, use eluents of similar viscosity when you run a gradient.
• Know the properties of your eluents. For example, cyclohexane solidifies
at 3.5 K psi.
• Consider column chemistry. If the column can be damaged by an
intermediate or new eluent, remove the column from the system and
substitute a union. See the care and usage guide for your column.
• Dedicate an eluent supply tube and filter assembly for each eluent. If
this is not possible, purge the tube and filter of any remaining eluent
before you use a new eluent.
• To prevent cross contamination of eluents, dedicate a set of glassware
for preparing, storing and supplying each eluent. Once a container has
been used for an eluent, do not use it for any other eluent unless it has
been thoroughly cleaned.
Warning: To avoid chemical hazards, observe safe laboratory practices
when you handle eluents. See the Material Safety Data Sheets for the
eluents you use. For more information on eluent properties, see
Appendix D, Eluent Considerations.
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