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Waters 515 HPLC - Page 55

Waters 515 HPLC
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Operating the pump 3-23
If your pump is connected to a Waters 680 Automated Gradient
Controller, the controller is powered on before you power on the pump.
Operating
To operate the pump in a remote configuration:
1. Press the Up, Down, or Menu key to advance past the Flow, HPL, and
LPL parameters. Since you set these parameters at the data system or
external controller, the local parameters at the pump are not used.
Tip: When the 515 pump is connected to a Waters 680 Automated
Gradient Controller, make sure the pump HPL parameter is set to 6000
psi (41370 kPa, 401 bars) and the pump Comp parameter is set to 0
(zero). In addition, you must set the flow rate, high-pressure limit, and
low-pressure limit at the controller.
2. Press Menu until the Mode parameter displays. If required, press the
Edit/Enter key, then the Up or Down key to set the parameter to Rem.
Press Menu to store the parameter and display the next menu item
(Comp).
3. If you need to change the Comp setting for your run, press Edit/Enter to
activate the blinking cursor, then use the Up or Down key to enter the
desired setting. See “Adjusting for eluent compressibility” on page 3-24
for instructions on how to adjust the Comp parameter for a change in
run conditions. When you finish, press Menu to store the parameter.
4. Press Menu until the Unit parameter appears. If you need to change the
Unit of pressure measurement, press Edit/Enter to activate the blinking
cursor, then use the Up or Down key to enter the desired setting. When
you finish, press Menu to store the parameter. The Unit selection does
not affect the units displayed at the remote device.
5. Once all parameters are correct, press Run/Stop. This places the pump
in the Run Rem mode, and the Flow area of the display reads No Flow.
The pump is ready to receive a remote run signal.
Warning: To avoid chemical hazards, observe safe laboratory practices
when you handle eluents. See the Material Safety Data Sheets for the
eluents you use. For more information on eluent properties, see
Appendix D, Eluent Considerations.

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