Troubleshooting 4-37
Baseline noise,
random
Eluents not properly
degassed or sparged
Degas/sparge eluents.
Flow erratic, pump not
primed
Prime the pump (see“Priming
the pump” on page 3-15).
Check for air in the pump,
failing seals.
Eluents contaminated Use fresh eluent.
Column contaminated Clean/replace column.
System improperly
grounded
Plug into outlet on different
electrical circuit.
Use power conditioner.
Recorder voltage
incorrect
Set recorder to correct voltage.
Radio frequency noise Eliminate interference.
Straight baseline, no
peaks
No pump flow Set pump flow rate.
Leak in eluent path Repair the leak.
Sensitivity loss Degraded,
contaminated, or
improperly prepared
sample
Use fresh sample.
Column contaminated Clean/replace column.
Loss of column
efficiency
Clean/replace column.
Change in eluent
composition
Correct eluent pH or ionic
composition.
Incorrect flow rate Change flow rate.
Identifying chromatographic problems (Continued)
Symptom Possible cause Corrective action
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