2Optimizing Performance
28 July 7, 2014, 715003736IVD Rev. C
Sample preparation
UPLC analysis places some additional restrictions on sample preparation.
Particulates
The small column frit size (0.2 µm) can become blocked more easily than
larger HPLC column frits (2.0 µm). Particle-free mobile phase solvents and
sample solutions are therefore essential for UPLC analysis. See “General
guidelines” on page 23 for recommendations on choosing and handling
solvents.
Matching sample diluents
When you use the auto-dilution option on the SM-FTN, the purge solvent
serves as the sample diluent. Ensure that your sample solution is soluble and
miscible in your chosen purge solvent.
Caution: To avoid broad peaks and poor peak shape, ensure that your
sample diluents match the initial conditions of your gradient.
To Next Page
Loading...