DSolvent Considerations
88 July 7, 2014, 715003736IVD Rev. C
Solvent miscibility
Before you change solvents, refer to the table below to determine solvent
miscibility. Be aware of these effects:
•Changes involving two miscible solvents can be made directly. Changes
involving two solvents that are not totally miscible (for example, from
chloroform to water) require an intermediate solvent like n-propanol.
•When you switch from a strong buffer to an organic solvent, thoroughly
flush the system using water before you add the organic solvent (see
“Water” on page 82).
Isobutyl alcohol 8.8 at 20 °C 107.7 28
Isopropyl alcohol 32.4 at 20 °C 82.26 12
Isopropyl myristate <1 at 20 °C 192.6 164
Methanol 97 at 20 °C 64.7 11
Methyl t-butyl ether 240 at 20 °C 55.2 -28
Methyl ethyl ketone 74 at 20 °C 79.64 -9
Methyl isobutyl ketone 16 at 20 °C 117.4 18
N-Methylpyrrolidone 0.33 at 25 °C 202.0 86
Pentane 420 at 20 °C 36.07 -49
n-Propyl alcohol 15 at 20 °C 97.2 23
Propylene carbonate 0.13 at 20 °C 241.7 135
Pyridine 18 at 25 °C 115.25 20
Toluene 28.5 at 20 °C 110.62 4
1,2,4-Trichlorobenzene 1 at 20 °C 213.5 106
Triethylamine 57 at 25 °C 89.5 -9
Trifluoroacetic acid 97.5 at 20 °C 71.8 -3
Water 17.54 at 20 °C 100.0
o-xylene 6 at 20 °C 144.41 17
Properties of common solvents: (Continued)
Solvent
Vapor Pressure
mm Hg (Torr)
Boiling
Point (°C)
Flash
Point (°C)
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