Solvent recommendations
July 7, 2014, 715003736IVD Rev. C 83
using a buffer, flush it from the pump by running a wet-prime with at least
five system volumes of HPLC-grade, distilled or deionized water.
To prevent microbial growth during shutdowns lasting longer than 24 h, flush
the pump and solvent lines with a solution of organic solvent/water in which
the organic component is greater than 20%.
Tip: To avoid salt precipitation, nonvolatile buffer concentrations must not
exceed 100 mM.
Solvent recommendations
The ACQUITY UPLC I-Class IVD system was designed for reversed-phase
chromatography and ACQUITY UPLC BEH column chemistries. Waters
evaluated the system’s reliability using traditional reversed-phase solvents.
This section lists solvents recommended for the ACQUITY UPLC I-Class IVD
system. Contact Waters Customer Service to determine whether you can use
solvents that do not appear in the list without adversely affecting instrument
or system performance.
Recommended solvents
•Acetonitrile
•Acetonitrile/water mixtures
•Isopropanol
•Methanol
•Methanol/water mixtures
•Water
Caution: To avoid damaging a mass spectrometer by unwittingly
introducing incompatible buffers, consult the instrument’s
accompanying documentation to determine what buffers are compatible.
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