DSolvent Considerations
86 July 7, 2014, 715003736IVD Rev. C
System recommendations
ACQUITY UPLC I-Class IVD system recommendations
•Contact Waters for recommended system cleaning and flushing
procedures.
•Contact your Waters sales representative or local technical support
organization to determine the typical chromatography conditions and/or
instrument operating parameters suitable for the ACQUITY I-Class
IVD system.
•Chloroform, methylene chloride, halogenated solvents, and toluene can
be used in weak dilutions (less than 10% vol.) as additives, modifiers, or
sample diluents.
•Methanesulfonic acid is not recommended for use in ACQUITY UPLC
I-Class IVD systems.
•Always use properly cleaned and maintained glassware.
Binary solvent manager recommendations
•The seal wash system must never run dry, particularly during
separations that use a polar mobile phase.
•For reversed-phase applications, use aqueous seal wash solutions with a
weak organic component (for example 1:9 methanol/water).
•Do not use nonvolatile buffers as seal wash solvents.
•Ensure that the mobile phase is completely soluble in and compatible
with all of the solvents in use on the system.
Caution: To prevent the proliferation of microbial colonies that clog
system filters and capillary lines, add a small amount (less than 10%) of
an organic solvent, such as acetonitrile or methanol, and completely
replace the mobile phase daily. Aqueous solvents must not remain in a
shut-down system because they serve as a substrate for microbial
growth.
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