OPERATION
ZEISS Illumination and contrast techniques Axio Observer
152 431004-7244-001 12/2016
(2) Transmitted light DIC with
30° analyzer slider (de SÉNARMONT)
This technique can only be applied using the 0.35 H/DIC condenser.
If the ±30° analyzer slider is used, the DIC slider must first be centered.
• Move the polarizer (Fig. 152/1) into position and adjust the ±30° analyzer (Fig. 152/5) to the 0° (dark)
position (polarizer and analyzer are perpendicular).
• Deselect the DIC prism on the condenser turret (e.g. use bright field or phase contrast).
• Remove one eyepiece and replace with the centering telescope (or swivel the Bertrand lens into
position on the phototube).
• If the field is viewed using the centering telescope (or Bertrand lens), a diagonal black line on the DIC
slider (left top to bottom right) will be visible.
• Move the diagonal black line to the center of the field of view by adjusting the knurled screw on the
DIC slider.
• Remove the centering telescope and reinsert the eyepiece (or move the Bertrand lens out of the
optical path).
• Select the DIC position on the condenser.
• Place the specimen onto the stage.
• Using the analyzer selector wheel, rotate the analyzer away from the 0° position until optimum
contrast is obtained.
Because the DIC technique uses polarized light, it will be disrupted if birefringent objects, e.g.
foils occasionally used with histological sections, are positioned between the polarizer and
analyzer. This problem may also arise with Plexiglas culture chambers if the chamber bottom is
made of plastic. In such ca
ses, it is advisable to use chambers with glass bottoms to avoid
impairment of the optical performance.
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