Agilent 1200 Preparative Pump User Manual 49
Installing the Pump
3
Get the System Ready for the First Analysis
Get the System Ready for the First Analysis
When you are using the system for the first time it is recommended to prime it
to remove all the air and the possible contamination introduced in the flow
path during the installation.
Priming your preparative LC system with the pump
1 Set the flow rate to 5 ml/min and pump for 5 minutes with an open EMPV
to prime your new tubings and capillaries and to remove potential air
bubbles or contamination from the flow path.
2 Check your system for leak tightness with the help of the built in pressure
test (see Pressure Test) (“Prep Pump Pressure Test Description” on
page 94).
3 Refer to “Optimizing Performance” on page 65 for optimizing your pump’s
performance.
4 Before starting an analysis pump until you receive a stable pressure signal
from your pump and until your detector baseline has stabilized (normally
no more than five minutes). Low %-ripple is a vital sign for a smoothly
operating pump and a stable system.
The pump should never be used for priming empty tubings (never let the pump run dry). Use
a syringe to draw enough solvent for completely filling the tubings to the pump inlet before
continuing to prime with the pump.
When opening capillary or tube fittings solvents may leak out.
The handling of toxic and hazardous solvents and reagents can hold health risks.
➔ Please observe appropriate safety procedures (for example, goggles, safety gloves
and protective clothing) as described in the material handling and safety data sheet
supplied by the solvent vendor, especially when toxic or hazardous solvents are
used.
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